SH2D1B antibody

Catalog No. ABIN7119007

This Rabbit Polyclonal antibody specifically detects SH2D1B in ELISA, IHC and WB. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for SH2D1B antibody (ABIN7119007)

Target: SH2D1B (SH2 Domain Containing 1B (SH2D1B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SH2D1B antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Product Details anti-SH2D1B Antibody

Purpose: SH2D1B antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: SH2 domain containing 1B

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IHC: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for SH2D1B

Target: SH2D1B (SH2 Domain Containing 1B (SH2D1B))

Alternative Name: SH2D1B

Background:

Synonyms: SH2 domain-containing protein 1B|EWS/FLI1-activated transcript 2 (EAT-2)|SH2D1B|EAT2

Background: Cytoplasmic adapter regulating receptors of the signaling lymphocytic activation molecule(SLAM) family such as CD84, SLAMF1, LY9 and CD244(PubMed:11689425). In SLAM signaling seems to cooperate with SH2D1A/SAP. Plays a role in regulation of effector functions of natural killer(NK) cells by controlling signal transduction through CD244/2B4 without effecting its tyrosine phosphorylation, downstream signaling involves PLCG1 and ERK activation(PubMed:24687958). Activation of SLAMF7-mediated NK cell function does not effect receptor tyrosine phosphorylation but distal signaling(By similarity). In the context of NK cell-mediated cytotoxicity does not enhance conjugate formation with target cells but stimulates polarization of the microtubule-organizing center and cytotoxic granules toward the NK cell synapse(PubMed:24687958). Negatively regulates CD40-induced cytokine procuction dendritic cells downstream of SLAM family receptors probably by inducing activation of the PI3K pathway to inhibit p38 MAPK and JNK activation(By similarity).

Molecular Weight: 15 kDa

Gene ID: 117157

UniProt: O14796

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process